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In Vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59

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In Vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59

Auteurs : Carol J. Budzilowicz [États-Unis] ; Susan R. Weiss [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1987.

RBID : ISTEX:4E6ECFC95B589738F8E9D61E6FB483B7C6535479

English descriptors

Teeft :
- Amino, Amino acid sequences, Amino acids, Amino terminus, Budzilowicz, Codon, Coronavirus, Eukaryotic mrnas, Gene, Genome, Hydropathy plots, Intergenic, Leibowitz, Mouse hepatitis virus, Mrna, Nonstructural, Nonstructural proteins, Nucleic acids, Nucleotide, Open reading frame, Open reading frames, Orfs, Pgem, Pgem vectors, Plasmid, Polymerase, Polypeptide, Protein synthesis, Putative, Reading frames, Rna, Sequencing, Siddell, Skinner, Spaan, Translation products, Viral, Viral genome, Weiss.

Abstract

Abstract: The complete nucleotide sequence of nonstructural gene 5 of coronavirus mouse hepatitis virus (MHV) strain A59 has been determined. This sequence contains two potential open reading frames which overlap by five nucleotides. The putative protein products predicted from the sequence are a basic 13,000-Da polypeptide and a 9600-Da polypeptide containing an unusually long hydrophobic amino terminus. RNAs transcribed in vitro from DNAs containing each of the open reading frames in pGEM vectors direct the synthesis in vitro of polypeptides of the sizes predicted by the sequence. An RNA transcript containing both of the open reading frames directs the synthesis primarily of the polypeptide corresponding to the downstream open reading frame. These data suggest that MHV-A59 messenger RNA 5 potentially encodes two proteins and may be preferentially translated from an internal AUG initiation codon.

Url:

https://api.istex.fr/ark:/67375/6H6-ZDSSPVXF-9/fulltext.pdf

DOI: 10.1016/0042-6822(87)90293-5

Affiliations:

États-Unis

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Le document en format XML

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<term>Codon</term>
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<term>Eukaryotic mrnas</term>
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<term>Mouse hepatitis virus</term>
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<term>Nonstructural proteins</term>
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<term>Nucleotide</term>
<term>Open reading frame</term>
<term>Open reading frames</term>
<term>Orfs</term>
<term>Pgem</term>
<term>Pgem vectors</term>
<term>Plasmid</term>
<term>Polymerase</term>
<term>Polypeptide</term>
<term>Protein synthesis</term>
<term>Putative</term>
<term>Reading frames</term>
<term>Rna</term>
<term>Sequencing</term>
<term>Siddell</term>
<term>Skinner</term>
<term>Spaan</term>
<term>Translation products</term>
<term>Viral</term>
<term>Viral genome</term>
<term>Weiss</term>
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<front><div type="abstract" xml:lang="en">Abstract: The complete nucleotide sequence of nonstructural gene 5 of coronavirus mouse hepatitis virus (MHV) strain A59 has been determined. This sequence contains two potential open reading frames which overlap by five nucleotides. The putative protein products predicted from the sequence are a basic 13,000-Da polypeptide and a 9600-Da polypeptide containing an unusually long hydrophobic amino terminus. RNAs transcribed in vitro from DNAs containing each of the open reading frames in pGEM vectors direct the synthesis in vitro of polypeptides of the sizes predicted by the sequence. An RNA transcript containing both of the open reading frames directs the synthesis primarily of the polypeptide corresponding to the downstream open reading frame. These data suggest that MHV-A59 messenger RNA 5 potentially encodes two proteins and may be preferentially translated from an internal AUG initiation codon.</div>
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In Vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59

In Vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri